CHIT1 (NM_001270509) Human Untagged Clone

CAT#: SC336019

CHIT1 (untagged) - Human chitinase 1 (chitotriosidase) (CHIT1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHIT1
• Synonyms: CHI3; CHIT; CHITD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001270509, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGCGGTCTGTGGCCTGGGCAGGTTTCATGGTCCTGCTGATGATCCCATGGGGCTCTGCTGCAAAAC
  TGGTCTGCTACTTCACCAACTGGGCCCAGTACAGACAGGGGGAGGCTCGCTTCCTGCCCAAGGACTTGGA
  CCCCAGCCTTTGCACCCACCTCATCTACGCCTTCGCTGGCATGACCAACCACCAGCTGAGCACCACTGAG
  TGGAATGACGAGACTCTCTACCAGGAGTTCAATGGCCTGAAGAAGATGAATCCCAAGCTGAAGACCCTGT
  TAGCCATCGGAGGCTGGAATTTCGGCACTCAGAAGTTCACAGATATGGTAGCCACGGCCAACAACCGTCA
  GACCTTTGTCAACTCGGCCATCAGGTTTCTGCGCAAATACAGCTTTGACGGCCTTGACCTTGACTGGGAG
  TACCCAGGAAGCCAGGGGAGCCCTGCCTTCCAGCAGGAAGCCCAGACCTCAGGGAAGGAACGCCTTCTTC
  TGAGTGCAGCGGTTCCAGCTGGGCAGACCTATGTGGATGCTGGATACGAGGTGGACAAAATCGCCCAGAA
  CCTGGATTTTGTCAACCTTATGGCCTACGACTTCCATGGCTCTTGGGAGAAGGTCACGGGACATAACAGC
  CCCCTCTACAAGAGGCAAGAAGAGAGTGGTGCAGCAGCCAGCCTCAACGTGGATGCTGCTGTGCAACAGT
  GGCTGCAGAAGGGGACCCCTGCCAGCAAGCTGATCCTTGGCATGCCTACCTACGGACGCTCCTTCACACT
  GGCCTCCTCATCAGACACCAGAGTGGGGGCCCCAGCCACAGGGTCTGGCACTCCAGGCCCCTTCACCAAG
  GAAGGAGGGATGCTGGCCTACTATGAAGTCTGCTCCTGGAAGGGGGCCACCAAACAGAGAATCCAGGATC
  AGAAGGTGCCCTACATCTTCCGGGACAACCAGTGGGTGGGCTTTGATGATGTGGAGAGCTTCAAAACCAA
  GGGCCGATACCCCCTCATCCAGACGCTACGGCAGGAACTGAGTCTTCCATACTTGCCTTCAGGCACCCCA
  GAGCTTGAAGTTCCAAAACCAGGTCAGCCCTCTGAACCTGAGCATGGCCCCAGCCCTGGACAAGACACGT
  TCTGCCAGGGCAAAGCTGATGGGCTCTATCCCAATCCTCGGGAACGGTCCAGCTTCTACAGCTGTGCAGC
  GGGGCGGCTGTTCCAGCAAAGCTGCCCGACAGGCCTGGTGTTCAGCAACTCCTGCAAATGCTGCACCTGG
  AATTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270509
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270509.1, NP_001257438.1
• RefSeq Size: 2190 bp
• RefSeq ORF: 1266 bp
• Locus ID: 1118
• Cytogenetics: 1q32.1
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Amino sugar and nucleotide sugar metabolism
• Gene Summary: 'Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (5) lacks an in-frame segment in the 3' coding region, compared to variant 1. The resulting isoform (3) is shorter, compared to isoform 1.