TFE3 (NM_001282142) Human Untagged Clone

CAT#: SC336310

TFE3 (untagged) - Human transcription factor binding to IGHM enhancer 3 (TFE3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TFE3
• Synonyms: bHLHe33; RCCP2; RCCX1; TFEA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001282142, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGTCATCTTCTTCATCGAGGGTCTTGCTGCGGCAGCAGCTAATGCGGGCCCAGGCGCAGGAGCAGG
  AGAGGCGTGAGCGTCGGGAACAGGCCGCCGCGGCTCCCTTCCCCAGTCCTGCACCTGCCTCTCCTGCCAT
  CTCTGTGGTTGGCGTCTCTGCTGGGGGCCACACATTGAGCCGTCCACCCCCTGCTCAGGTGCCCAGGGAG
  GTGCTCAAGGTGCAGACCCATCTGGAGAACCCAACGCGCTACCACCTGCAGCAGGCGCGCCGGCAGCAGG
  TGAAACAGTACCTGTCCACCACACTCGGGCCCAAGCTGGCTTCCCAGGCCCTCACCCCACCGCCGGGGCC
  CGCAAGTGCCCAGCCACTGCCTGCCCCTGAGGCTGCCCACACTACCGGCCCCACAGGCAGTGCGCCCAAC
  AGCCCCATGGCGCTGCTCACCATCGGGTCCAGCTCAGAGAAGGAGATTGATGATGTCATTGATGAGATCA
  TCAGCCTGGAGTCCAGTTACAATGATGAAATGCTCAGCTATCTGCCCGGAGGCACCACAGGACTGCAGCT
  CCCCAGCACGCTGCCTGTGTCAGGGAATCTGCTTGATGTGTACAGTAGTCAAGGCGTGGCCACACCAGCC
  ATCACTGTCAGCAACTCCTGCCCAGCTGAGCTGCCCAACATCAAACGGGAGATCTCTGAGACCGAGGCAA
  AGGCCCTTTTGAAGGAACGGCAGAAGAAAGACAATCACAACCTAATTGAGCGTCGCAGGCGATTCAACAT
  TAACGACAGGATCAAGGAACTGGGCACTCTCATCCCTAAGTCCAGTGACCCGGAGATGCGCTGGAACAAG
  GGCACCATCCTGAAGGCCTCTGTGGATTATATCCGCAAGCTGCAGAAGGAGCAGCAGCGCTCCAAAGACC
  TGGAGAGCCGGCAGCGATCCCTGGAGCAGGCCAACCGCAGCCTGCAGCTCCGAATTCAGGAACTAGAACT
  GCAGGCCCAGATCCATGGCCTGCCAGTACCTCCCACTCCAGGGCTGCTTTCCTTGGCCACGACTTCGGCT
  TCTGACAGCCTCAAGCCAGAGCAGCTGGACATTGAGGAGGAGGGCAGGCCAGGCGCAGCAACGTTCCATG
  TAGGGGGGGGACCTGCCCAGAATGCTCCCCATCAGCAGCCCCCTGCACCGCCCTCAGATGCCCTTCTGGA
  CCTGCACTTTCCCAGCGACCACCTGGGGGACCTGGGAGACCCCTTCCACCTGGGGCTGGAGGACATTCTG
  ATGGAGGAGGAGGAGGGGGTGGTGGGAGGACTGTCGGGGGGTGCCCTGTCCCCACTGCGGGCTGCCTCCG
  ATCCCCTGCTCTCTTCAGTGTCCCCTGCTGTCTCCAAGGCCAGCAGCCGCCGCAGCAGCTTCAGCATGGA
  AGAGGAGTCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282142
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282142.1, NP_001269071.1
• RefSeq Size: 3402 bp
• RefSeq ORF: 1413 bp
• Locus ID: 7030
• Cytogenetics: Xp11.23
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.