Calpastatin (CAST) (NM_001284212) Human Untagged Clone

CAT#: SC337157

CAST (untagged) - Human calpastatin (CAST), transcript variant 13

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAST
• Synonyms: BS-17; PLACK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001284212, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATCCCACAGAAACCAAGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCCAAAAA
  GCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCAGC
  TGAACAGCAGCCATCAGAGAAATCAACAGAACCAAAGACTAAACCACAAGACATGATTTCTGCTGGTGGA
  GAGAGTGTTGCTGGTATCACTGCAATATCTGGCAAGCCGGGTGACAAGAAAAAAGAAAAGAAATCATTAA
  CCCCAGCTGTGCCAGTTGAATCTAAACCGGATAAACCATCGGGAAAGTCAGGCATGGATGCTGCTTTGGA
  TGACTTAATAGATACTTTAGGAGGACCTGAAGAAACTGAAGAAGAAAATACAACGTATACTGGACCAGAA
  GTTTCAGATCCAATGAGTTCCACCTACATAGAGGAATTGGGTAAAAGAGAAGTCACAATTCCTCCAAAAT
  ATAGGGAACTATTGGCTAAAAAGGAAGGGATCACAGGGCCTCCTGCAGACTCTTCGAAACCCATAGGGCC
  AGATGATGCTATAGACGCCTTGTCATCTGACTTCACCTGTGGGTCGCCTACAGCTGCTGGAAAGAAAACT
  GAAAAAGAGGAATCTACAGAAGTTTTAAAAGCTCAGTCAGCAGGGACAGTCAGAAGTGCTGCTCCACCCC
  AAGAGAAGAAAAGAAAGGTGGAGAAGGATACAATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCACT
  GGGCACCCGGCAAGCAGAACCTGAGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAGGCAAAAGCTAAA
  GAAGAAAAACTAGAGAAGTGTGGTGAGGATGATGAAACAATCCCATCTGAGTACAGATTAAAACCAGCCA
  CGGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAAGAAAAACCCAAGCCTCGGAGTGAATCAGAACT
  CATTGATGAACTTTCAGAAGATTTTGACCGGTCTGAATGTAAAGAGAAACCATCTAAGCCAACTGAAAAG
  ACAGAAGAATCTAAGGCCGCTGCTCCAGCTCCTGTGTCGGAGGCTGTGTGTCGGACCTCCATGTGTAGTA
  TACAGTCAGCACCCCCTGAGCCGGCTACCTTGAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTGGC
  TGATAGCCTGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCTGTGATGGATAAAGTCAAGGAGAAG
  GCCAAAGAAGAAGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATTCCTCCTGATTATAGATTAGAAG
  AGGTCAAGGATAAAGATGGAAAGCCACTCCTGCCAAAAGAGTCTAAGGAACAGCTTCCACCCATGAGTGA
  AGACTTCCTTCTGGATGCTTTGTCTGAGGACTTCTCTGGTCCACAAAATGCTTCATCTCTTAAATTTGAA
  GATGCTAAACTTGCTGCTGCCATCTCTGAAGTGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCTGGAG
  CCCCACCCCGTGATACCTCGAGTGACAAAGACCTCGATGATGCCTTGGATAAACTCTCTGACAGTCTAGG
  ACAAAGGCAGCCTGACCCAGATGAGAACAAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAAGCTGAA
  CATAGAGACAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAATACAGACATCTCCTGGATGATAATG
  GACAGGACAAACCAGTGAAGCCACCTACAAAGAAATCAGAGGATTCAAAGAAACCTGCAGATGACCAAGA
  CCCCATTGATGCTCTCTCAGGAGATCTGGACAGCTGTCCCTCCACTACAGAAACCTCACAGAACACAGCA
  AAGGATAAGTGCAAGAAGGCTGCTTCCAGCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAGGATTCAG
  CAAAGACAACAGAGGAAACTTCCAAGCCAAAAGATGACTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284212
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284212.2, NP_001271141.1
• RefSeq Size: 2392 bp
• RefSeq ORF: 2001 bp
• Locus ID: 831
• Cytogenetics: 5q15
• Gene Summary: 'The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]'