BRD2 (NM_001291986) Human Untagged Clone

CAT#: SC337191

BRD2 (untagged) - Human bromodomain containing 2 (BRD2), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRD2
• Synonyms: BRD2-IT1; D6S113E; FSH; FSRG1; NAT; O27.1.1; RING3; RNF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001291986, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACATGGGTACTATTAAGAGGAGACTTGAAAACAATTATTATTGGGCTGCTTCAGAGTGTATGCAAG
  ATTTTAATACCATGTTCACCAACTGTTACATTTACAACAAGCCCACTGATGATATTGTCCTAATGGCACA
  AACGCTGGAAAAGATATTCCTACAGAAGGTTGCATCAATGCCACAAGAAGAACAAGAGCTGGTAGTGACC
  ATCCCTAAGAACAGCCACAAGAAGGGGGCCAAGTTGGCAGCGCTCCAGGGCAGTGTTACCAGTGCCCATC
  AGGTGCCTGCCGTCTCTTCTGTGTCACACACAGCCCTGTATACTCCTCCACCTGAGATACCTACCACTGT
  CCTCAACATTCCCCACCCATCAGTCATTTCCTCTCCACTTCTCAAGTCCTTGCACTCTGCTGGACCCCCG
  CTCCTTGCTGTTACTGCAGCTCCTCCAGCCCAGCCCCTTGCCAAGAAAAAAGGCGTAAAGCGGAAAGCAG
  ATACTACCACCCCTACACCTACAGCCATCTTGGCTCCTGGTTCTCCAGCTAGCCCTCCTGGGAGTCTTGA
  GCCTAAGGCAGCACGGCTTCCCCCTATGCGTAGAGAGAGTGGTCGCCCCATCAAGCCCCCACGCAAAGAC
  TTGCCTGACTCTCAGCAACAACACCAGAGCTCTAAGAAAGGAAAGCTTTCAGAACAGTTAAAACATTGCA
  ATGGCATTTTGAAGGAGTTACTCTCTAAGAAGCATGCTGCCTATGCTTGGCCTTTCTATAAACCAGTGGA
  TGCTTCTGCACTTGGCCTGCATGACTACCATGACATCATTAAGCACCCCATGGACCTCAGCACTGTCAAG
  CGGAAGATGGAGAACCGTGATTACCGGGATGCACAGGAGTTTGCTGCTGATGTACGGCTTATGTTCTCCA
  ACTGCTATAAGTACAATCCCCCAGATCACGATGTTGTGGCAATGGCACGAAAGCTACAGGATGTATTTGA
  GTTCCGTTATGCCAAGATGCCAGATGAACCACTAGAACCAGGGCCTTTACCAGTCTCTACTGCCATGCCC
  CCTGGCTTGGCCAAATCGTCTTCAGAGTCCTCCAGTGAGGAAAGTAGCAGTGAGAGCTCCTCTGAGGAAG
  AGGAGGAGGAAGATGAGGAGGACGAGGAGGAAGAAGAGAGTGAAAGCTCAGACTCAGAGGAAGAAAGGGC
  TCATCGCTTAGCAGAACTACAGGAACAGCTTCGGGCAGTACATGAACAACTGGCTGCTCTGTCCCAGGGT
  CCAATATCCAAGCCCAAGAGGAAAAGAGAGAAAAAAGAGAAAAAGAAGAAACGGAAGGCAGAGAAGCATC
  GAGGCCGAGCTGGGGCCGATGAAGATGACAAGGGGCCTAGGGCACCCCGCCCACCTCAACCTAAGAAGTC
  CAAGAAAGCAAGTGGCAGTGGGGGTGGCAGTGCTGCTTTAGGCCCTTCTGGCTTTGGACCTTCTGGAGGA
  AGTGGCACCAAGCTCCCCAAAAAGGCCACAAAGACAGCCCCACCTGCCCTGCCTACAGGTTATGATTCAG
  AGGAGGAGGAAGAGAGCAGGCCCATGAGTTACGATGAGAAGCGGCAGCTGAGCCTGGACATCAACAAATT
  ACCTGGGGAGAAGCTGGGCCGAGTTGTGCATATAATCCAAGCCAGGGAGCCCTCTTTACGTGATTCAAAC
  CCAGAAGAGATTGAGATTGATTTTGAAACACTCAAGCCATCCACACTTAGAGAGCTTGAGCGCTATGTCC
  TTTCCTGCCTACGTAAGAAACCCCGGAAGCCCTACACCATTAAGAAGCCTGTGGGAAAGACAAAGGAGGA
  ACTGGCTTTGGAGAAAAAGCGGGAATTAGAAAAGCGGTTACAAGATGTCAGCGGACAGCTCAATTCTACT
  AAAAAGCCCCCCAAGAAAGCGAATGAGAAAACAGAGTCATCCTCTGCACAGCAAGTAGCAGTGTCACGCC
  TTAGCGCTTCCAGCTCCAGCTCAGATTCCAGCTCCTCCTCTTCCTCGTCGTCGTCTTCAGACACCAGTGA
  TTCAGACTCAGGCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291986
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291986.1, NP_001278915.1
• RefSeq Size: 4899 bp
• RefSeq ORF: 2046 bp
• Locus ID: 6046
• Cytogenetics: 6p21.32
• Protein Families: Protein Kinase
• Gene Summary: 'This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010]'
  Transcript Variant: This variant (5) has an alternate 5' exon and an additional internal exon compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.