NS1 (NC_012042) Virus Tagged ORF Clone

CAT#: VC100544

Myc-DDK-tagged ORF clone of viral ORF for NS1 [Human bocavirus 2], codon optimized for human cell expression, YP_002586773

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100544 represents NCBI reference of YP_002586773 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTCTCCGCACCTGTAATAAGGGCATTCTCTCAGCCAGCATTTACTTATGTCGTTAAATTCCCAT
  ATGAGAATTGGAAAGAGGAAGAGCACCTTCTGTGGAGCCTTCTGGCCCCAGGGACCGAAAGGCTGATGAT
  CCAGCTCCGAAATTGCGCCCCTCATCCCGAAGACGATCCAGTCCGAGAGGACATACTGTGCTCTCTTGCA
  GACCAGCACTATGGAGCAATCTTTGCAAAAGCTTGCTACATTGCTACAACAACCCTCATGGGCCAAAAGC
  AACGCACCCCATTTCCTCGGTGCGATATCATATGCCAATCAGAGATCGGTTCAGAGCACCTGCATTGTCA
  CATACTCGTGGGCGGGGCCGGACTCAGTAAAAGAAATGCGAAGATATCACGAGCCACCCTTCTCGGCCTT
  GTCATGGCTGAACTGACACAGCGGTGCAAACAGTTGCTTGCATTGAGACCTTTCGAGCCCGCCGAAGCTA
  ATATTTTCCACCTGCTTAAACGAATTGAGAGAGAAGCTTGGAGTGGGCACACTGGTAACTGGGTGCAGAT
  TCTTCAGTATAAGGACAAAAGGGGTGATCTCCATGCCCAACCAATTGACCCCCTGAGATTCCTCAAGCAT
  TACATCCTCCCCAAGAATAGGCTTATTTCTCCAAGCAGCAAGCCTGACGTGTGCACAACCCCAGACAACT
  GGTTTATCCTGGCTGACAAGACCTACGCCCACACTATAATCAACGGCCTCCCCTTGCTGGAACACAATCG
  GAAGGCCTACCTCCAGGAGCTCGAGTCCGAGGTGATCCCAGGGCCTTCTACAATGGCTTTTGGAGGTAGG
  GGGGCGTGGGAGCAGCTGCCCGAGGTGGGCGAGCAACGCTTGATAACAAGCAACGCTAGCACCGCATACA
  AAGCCAATAAGAAAGAGAAGCTGATGCTGAATTTGCTGGATAAGTGCGACGAACTGAATCTGCTGGTCTA
  TGAGGACCTGGTGTCTGCCTGCCCTGACCTGCTCCTCATGCTTGAGGGCCAACCTGGCGGCGCTCGACTC
  ATCGAACAAGTGTTGGGCATGCATCACATAAAAGTTTGCGCAAAATATACCGCCCTTACCTTCTTGTTCC
  ACTTGCATCCTGATCAGCTGCTGACTTCTAACAACAAAGCTCTGAAACTGCTCCTGATACAGGGATACAA
  CCCACTTCAGGTAGGACACGCGATCTGCTGTGTGCTCAATAAGCAGATGGGAAAGCAGAATACTATCTGT
  TTTTACGGACCTGCTTCCACCGGCAAGACCAATTTTGCAAAGGCCATCGTGCAGGGCGTGAGGCTGTATG
  GATGTGTGAACCACCTCAATAAGGGTTTTGTCTTTAATGATTGCCGGCAGCGACTGATAATCTGGTGGGA
  GGAGTGTTTGATGCACCAAGATTGGGTGGAGCCCGCTAAATGTATCCTGGGCGGCACTGAATGTAGGATT
  GATGTGAAGCACAAGGATTCCGTTCTGCTCCAGCAAACGCCTGTGATCATAAGTACTAACCATGACATTT
  ACTCTGTAGTCGGCGGAAACACTGTGTCCCATGTCCATGCTGCTCCCCTGAAGGAAAGGGTACTTCAGCT
  CAACTTTATGAAGCAACTGCCTCAGACCTTTGGCGAAATCAGCCCCGTGGAAATCGCCGAACTGCTCCAG
  TGGTGCTTCAATGAGTACGAGTGCACGCTGACCGGATTTAAACAGAAATGGAACCTCGACAAAGTGCCCA
  ATTCCTTTCCCCTGGGCGACCTCTGCCCTACCCACAGCCAGGACTACACACTGCACGAGAATGGGTTCTG
  CACTGACTGCGGCGGCTATCTGCCCCATTCTGCTGACGACTTCGTATACACCGATGTGGCCTCAGAGACT
  ACCAGTGGCGATTGCGATCCAGGGCGACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100544 representing YP_002586773
  Red=Cloning sites Green=Tags
  
  MAFSAPVIRAFSQPAFTYVVKFPYENWKEEEHLLWSLLAPGTERLMIQLRNCAPHPEDDPVREDILCSLA
  DQHYGAIFAKACYIATTTLMGQKQRTPFPRCDIICQSEIGSEHLHCHILVGGAGLSKRNAKISRATLLGL
  VMAELTQRCKQLLALRPFEPAEANIFHLLKRIEREAWSGHTGNWVQILQYKDKRGDLHAQPIDPLRFLKH
  YILPKNRLISPSSKPDVCTTPDNWFILADKTYAHTIINGLPLLEHNRKAYLQELESEVIPGPSTMAFGGR
  GAWEQLPEVGEQRLITSNASTAYKANKKEKLMLNLLDKCDELNLLVYEDLVSACPDLLLMLEGQPGGARL
  IEQVLGMHHIKVCAKYTALTFLFHLHPDQLLTSNNKALKLLLIQGYNPLQVGHAICCVLNKQMGKQNTIC
  FYGPASTGKTNFAKAIVQGVRLYGCVNHLNKGFVFNDCRQRLIIWWEECLMHQDWVEPAKCILGGTECRI
  DVKHKDSVLLQQTPVIISTNHDIYSVVGGNTVSHVHAAPLKERVLQLNFMKQLPQTFGEISPVEIAELLQ
  WCFNEYECTLTGFKQKWNLDKVPNSFPLGDLCPTHSQDYTLHENGFCTDCGGYLPHSADDFVYTDVASET
  TSGDCDPGRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012042
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012042.1, YP_002586773
• RefSeq ORF: 1920
• Locus ID: 7755626
• MW: 72.1 kDa