NP1 (NC_012042) Virus Tagged ORF Clone

CAT#: VC100545

Myc-DDK-tagged ORF clone of viral ORF for NP1 [Human bocavirus 2], codon optimized for human cell expression, YP_002586774

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100545 represents NCBI reference of YP_002586774 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCAGAATCTATGAAGAATAAACATAGAAATTCTAAACGGACCCCTAGCCCTCTGCAAAAGGAAC
  GGAGACGGAACTGGGAAAGTCATAAATCTAGGTCCCGGTCTCCAATCCGAAGGCATGGAGAGAAGAACCT
  TGAGTATGCCCATCACTCTAATCAGGAAAACCGCCAGTCATCCTACACCGCGCTCAAGACCTCAGATCAG
  GCCACCAAAACAAAGGAAAAAACTTCCGGTGGCAATCGGACTAATCCTTACACAGTATTCTCTCAACATC
  GCGCGAACCATAGCGATGCTCCCGGCTGGTGTGGCTTCTACTGGCACAGCACACGGTTGGCAAGAGATGG
  GACGAACTGCATTTTCAACGAAATGAAACAGGAGTTTCAGGAACTGCAGATTAACGGGAAGATTACATGG
  GACAACGCACGAGAATTGCTTTTTTCTCAGAAAAAAAAGCTTGACCAGAAATACCGGAACATGCTGTATC
  ATTTTCGACACAGTCCTGACTGCCCTCGCTGCGACTACTGGGATAATGTCTATAGGCGGCACCTTGCTCA
  TGTATCCAGCCAAGAGTCAGAAGAGGTGACAGACGAGGAGATGCTGAGTGCTGTCGAGAGTATGGACACT
  AACGCCAGCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100545 representing YP_002586774
  Red=Cloning sites Green=Tags
  
  MSSESMKNKHRNSKRTPSPLQKERRRNWESHKSRSRSPIRRHGEKNLEYAHHSNQENRQSSYTALKTSDQ
  ATKTKEKTSGGNRTNPYTVFSQHRANHSDAPGWCGFYWHSTRLARDGTNCIFNEMKQEFQELQINGKITW
  DNARELLFSQKKKLDQKYRNMLYHFRHSPDCPRCDYWDNVYRRHLAHVSSQESEEVTDEEMLSAVESMDT
  NASN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012042
• ORF Size: 642 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012042.1, YP_002586774
• RefSeq ORF: 642
• Locus ID: 7755623
• MW: 25.3 kDa