NS1 (NC_012564) Virus Tagged ORF Clone

CAT#: VC100548

Myc-DDK-tagged ORF clone of viral ORF for NS1 [Human bocavirus 3], codon optimized for human cell expression, YP_002808454

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100548 represents NCBI reference of YP_002808454 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTTAACCCACCCGTAATCAGAGCCTTCTCATCCCCAGCCTTTACATATGTTTTTAAGTTCCCAT
  ATCCATCCTGGAAGGAAAAAGAGTGGCTCCTTCATGCACTCCTCGCACATGGCACTGAGCAGGCTATGAT
  TCAGCTGCGGAACTGCGTTCCTCACCCTGACGAAGACATCATTAGAGACGATCTTCTGCTCAGTCTGGAA
  GACCGCCATTTTGGGGCTATTCTGTGTAAGGCAGTGTATATGGCAACTACTACCTTTATGTCCCAGAAGC
  AGAGAAATATGTTTCCACGGTGCGATATTATCGTCCAATCCGAGCTCGGTGAGACAAATCTGCACTGCCA
  CATAATAGTTGGGGGTGAAGGCCTGTCCAAGAGAAACGCTAAAACATCATGCCCTCAGCTGTATGGACTG
  ATTTTGGGAGAACTGATACAGAGGTGCAAGACACTGCTGGCTACCCGCCCCTTTGAGCCCGAGGAGGCGG
  AAATTTACCACGCACTGAAGAGGGCTGAGCGCGAGGCCTGGGGGGGGGTCACGTCAGGGAACCTGCAGAT
  TTTGCAGTACAGAGACAGACGCGGCGACCTCCATGCTCAGCAGGTGGATGCCCTCAGATTTTTTAAAAAT
  TACCTGCTGCCTAAGAACCGATGTATTACCAGCTACAGCCGGCCCGACGTCTGTACGTCCCCCGAAAACT
  GGTTCGTTCTGGCTGAGAAGACGTACTGTCACACACTGGTTAACGGCCTGCCACTCCCGGAACACTACCG
  CAAACATTACCACGCTACTCTCGACAATGAGGTTCTGCCTGGACCTCAGACAATGGCCTTTGGCGGCAGA
  GGCCCTTGGGAACATCTGCCAGAGGTTGGGGACCAGAGACTTGCAGCTTCTTCCGTGTCCACAACATACA
  AACCTAACAAGAAAGAAAAGCTCATGCTGAACCTGCTGGACAAATGCTCAGAATTGAACCTGCTCGTATA
  TGAAGACCTCGTAGCAAACTGCCCAGAGCTGCTTCTCATGCTCGAGGGACAGCCCGGAGGGGCTAGACTG
  ATCGAACAGGTGCTGGGTATGCACCACATTAACGTGTGTAGTAATTTCACTGCCCTGAGCTATCTGTTCC
  ACCTGTACCCCGGTACAACACTTTCAAGCGATAATAAGGCCTTGCAACTGCTGCTGATTCAGGGTTACAA
  CCCACTGATGGTAGGCCACGCCCTCTGCTGTGTTCTGAATAAACAGTTCGGAAAGCAGAACACAGTCTGT
  TTTTATGGACCGGCCTCAACTGGCAAAACCAACATGGCCAAAGCTATCGTCCAAGGAATCCGGCTCTACG
  GGTGTGTGAACCACCTGAATAAAGGCTTCGTCTTCAATGATTGCAGGCAGCGCCTGGTGGTGTGGTGGGA
  AGAGTGTCTGATGCATCAGGACTGGGTTGAGCCGGCCAAGTGTATTCTGGGTGGAACAGAATGCCGGATT
  GACGTGAAGCATCGGGATAGCGTGCTCCTGACCCAAACCCCCGTTATCATCTCCACCAATCACGATATCT
  ATGCAGTAGTGGGGGGTAATTCCGTAAGCCACGTGCACGCGGCCCCCCTTAAGGAGCGGGTAATCCAACT
  GAACTTTATGAAACAACTGCCACAGACCTTTGGCGAGATCACACCAGAAGAAATAGCCGCTCTCCTTCAA
  TGGTGTTTTAACGAATACGAGTGCACTCTGACTGGCTTCAAGACCAAGTGGAGCCTGGACAAGATTCCCA
  ACTCTTTCCCCCTGGGAGTGCTGTGCCCCACACACTCACAAGACTTCATTCTCCACGAAAATGGCTACTG
  TACCGACTGTGGCGGATATCTGGCGCACTCCGCCGACGACTCAGTGTACACAGACCGGGCAAGTGATACA
  AGCAAGGAAGCTATTGATGCGGGCAAATTCACCTTTAGCAAGCACTTCATTTATATCCTGTACACAACCT
  TGAAGCACATGTTTAATTATCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100548 representing YP_002808454
  Red=Cloning sites Green=Tags
  
  MAFNPPVIRAFSSPAFTYVFKFPYPSWKEKEWLLHALLAHGTEQAMIQLRNCVPHPDEDIIRDDLLLSLE
  DRHFGAILCKAVYMATTTFMSQKQRNMFPRCDIIVQSELGETNLHCHIIVGGEGLSKRNAKTSCPQLYGL
  ILGELIQRCKTLLATRPFEPEEAEIYHALKRAEREAWGGVTSGNLQILQYRDRRGDLHAQQVDALRFFKN
  YLLPKNRCITSYSRPDVCTSPENWFVLAEKTYCHTLVNGLPLPEHYRKHYHATLDNEVLPGPQTMAFGGR
  GPWEHLPEVGDQRLAASSVSTTYKPNKKEKLMLNLLDKCSELNLLVYEDLVANCPELLLMLEGQPGGARL
  IEQVLGMHHINVCSNFTALSYLFHLYPGTTLSSDNKALQLLLIQGYNPLMVGHALCCVLNKQFGKQNTVC
  FYGPASTGKTNMAKAIVQGIRLYGCVNHLNKGFVFNDCRQRLVVWWEECLMHQDWVEPAKCILGGTECRI
  DVKHRDSVLLTQTPVIISTNHDIYAVVGGNSVSHVHAAPLKERVIQLNFMKQLPQTFGEITPEEIAALLQ
  WCFNEYECTLTGFKTKWSLDKIPNSFPLGVLCPTHSQDFILHENGYCTDCGGYLAHSADDSVYTDRASDT
  SKEAIDAGKFTFSKHFIYILYTTLKHMFNYR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012564
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012564.1, YP_002808454
• RefSeq ORF: 1983
• Locus ID: 7768240
• MW: 74.9 kDa