VP2 (NC_004295) Virus Tagged ORF Clone

CAT#: VC100784

Myc-DDK-tagged ORF clone of viral ORF for VP2 protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694867

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100784 represents NCBI reference of NP_694867 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTCAGTGAACAGCGCCGAAGCCAGTACCGGAGCTGGAGGAGGAGGATCAAACCCGACTAAGTCTA
  TGTGGAGTGAAGGTGCAACCTTCACCGCCAATTCCGTCACCTGTACCTTTAGCAGGCAGTTCCTGATTCC
  CTACGACCCAGAACATCATTATAAGGTGTTCTCACCGGCGGCCAGTAGCTGTCATAATGCTTCCGGTAAG
  GAAGCAAAGGTGTGCACTATCAGCCCAATTATGGGCTATAGTACGCCATGGCGCTACCTCGACTTCAACG
  CCTTGAATCTGTTCTTTAGTCCACTGGAGTTTCAGCACCTGATCGAGAATTACGGCAGCATCGCTCCTGA
  CGCACTGACAGTAACGATTAGTGAGATCGCCGTCAAGGACGTTACGGATAAGACGGGCGGAGGTGTCCAG
  GTCACCGACAGTACAACGGGGAGACTGTGTATGCTTGTAGATCACGAATATAAATATCCCTATGTGCTGG
  GGCAAGGTCAGGATACATTGGCCCCTGAGTTGCCTATTTGGGTATATTTCCCACCCCAGTATGCTTACCT
  GACGGTGGGCGAGGTCAATACGCAGGGAATCAGCGGAGATTCTAAGAAACTTGCCTCAGAAGAATCAGCC
  TTTTACGTTCTCGAGCACAGCTCCTTCGAGCTCCTGGGTACCGGTGGCAGTGCGACAATGTCTTACAAAT
  TCCCTGCTGTGCCCCCTGAGAACTTGGAGGGCTGTTCCCAGCATTTTTACGAGATGTACAACCCTCTGTA
  CGGCAGTCGATTGGGCGTTCCAGATACACTGGGTGGCGATCCTAAATTTCGCAGCCTTACCCATGAAGAC
  CATGCCATTCAACCGCAGAACTTTATGCCGGGGCCTCTCATTAATTCCGTTTCAACAAAAGAGGGGGACA
  ATTCCAACACTGGGGCCGGGAAGGCGCTGACAGGCCTGAGCACAGGGACTTCTCAAAATACCAGGATCAG
  CCTGCGGCCAGGGCCTGTGAGTCAGCCCTATCACCACTGGGATACCGATAAATATGTCACCGGCATCAAC
  GCCATTTCACACGGACAAACAACATACGGCAATGCCGAGGACAAGGAGTACCAACAGGGAGTGGGACGAT
  TTCCAAATGAGAAGGAACAACTGAAGCAGCTGCAGGGCCTGAATATGCACACATATTTTCCAAACAAGGG
  TACACAACAGTACACCGACCAGATTGAGCGGCCTCTGATGGTGGGATCTGTGTGGAACAGACGGGCGTTG
  CACTACGAGTCCCAGCTGTGGTCTAAGATCCCGAATCTCGATGATTCATTCAAAACCCAGTTCGCCGCCC
  TCGGCGGATGGGGCCTGCATCAGCCCCCACCCCAGATATTCCTGAAGATCCTGCCGCAGTCTGGGCCGAT
  TGGAGGGATCAAAAGTATGGGAATTACTACTCTGGTTCAGTACGCAGTGGGTATCATGACTGTAACAATG
  ACGTTCAAACTGGGTCCGCGCAAGGCTACTGGTAGGTGGAACCCACAGCCCGGAGTATACCCACCGCACG
  CCGCTGGCCACCTGCCTTATGTGCTGTACGATCCTACCGCAACAGACGCTAAGCAGCACCACCGCCATGG
  TTACGAAAAACCGGAGGAGCTGTGGACCGCTAAATCCCGCGTGCATCCGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100784 representing NP_694867
  Red=Cloning sites Green=Tags
  
  MTSVNSAEASTGAGGGGSNPTKSMWSEGATFTANSVTCTFSRQFLIPYDPEHHYKVFSPAASSCHNASGK
  EAKVCTISPIMGYSTPWRYLDFNALNLFFSPLEFQHLIENYGSIAPDALTVTISEIAVKDVTDKTGGGVQ
  VTDSTTGRLCMLVDHEYKYPYVLGQGQDTLAPELPIWVYFPPQYAYLTVGEVNTQGISGDSKKLASEESA
  FYVLEHSSFELLGTGGSATMSYKFPAVPPENLEGCSQHFYEMYNPLYGSRLGVPDTLGGDPKFRSLTHED
  HAIQPQNFMPGPLINSVSTKEGDNSNTGAGKALTGLSTGTSQNTRISLRPGPVSQPYHHWDTDKYVTGIN
  AISHGQTTYGNAEDKEYQQGVGRFPNEKEQLKQLQGLNMHTYFPNKGTQQYTDQIERPLMVGSVWNRRAL
  HYESQLWSKIPNLDDSFKTQFAALGGWGLHQPPPQIFLKILPQSGPIGGIKSMGITTLVQYAVGIMTVTM
  TFKLGPRKATGRWNPQPGVYPPHAAGHLPYVLYDPTATDAKQHHRHGYEKPEELWTAKSRVHPL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004295
• ORF Size: 1662 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004295.1, NP_694867
• RefSeq ORF: 1662
• Locus ID: 955442
• MW: 60.9 kDa