capsid (NC_001802) Virus Tagged ORF Clone

CAT#: VC101725

Myc-DDK-tagged ORF clone of viral ORF for capsid [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579880

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: capsid
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101725 represents NCBI reference of NP_579880 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAATAGTTCAGAATATCCAAGGCCAAATGGTCCATCAGGCGATCTCCCCCCGGACACTGAACGCCT
  GGGTAAAGGTAGTCGAGGAGAAGGCATTTAGCCCTGAGGTCATACCCATGTTTAGCGCCCTGTCTGAGGG
  GGCTACTCCTCAGGACTTGAACACTATGCTCAATACCGTCGGGGGACACCAAGCCGCTATGCAGATGCTC
  AAAGAAACTATCAATGAAGAAGCCGCTGAGTGGGATCGCGTCCATCCCGTTCATGCAGGTCCTATAGCGC
  CAGGTCAGATGAGAGAACCTAGAGGCAGCGATATCGCTGGCACCACCTCCACTCTTCAGGAGCAGATAGG
  CTGGATGACTAATAACCCCCCGATCCCCGTTGGGGAGATTTATAAGAGATGGATTATCCTGGGCCTTAAC
  AAGATCGTACGGATGTATAGCCCTACTAGTATCCTGGATATCAGACAGGGACCTAAGGAGCCCTTTCGGG
  ACTATGTTGACAGATTTTATAAGACACTCCGCGCTGAGCAGGCCTCCCAGGAAGTGAAAAATTGGATGAC
  TGAAACTCTTCTGGTGCAGAACGCCAATCCCGACTGTAAGACCATCCTGAAGGCATTGGGACCAGCTGCT
  ACACTCGAAGAGATGATGACTGCATGCCAGGGCGTGGGAGGACCAGGACACAAGGCCAGAGTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101725 representing NP_579880
  Red=Cloning sites Green=Tags
  
  MPIVQNIQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQML
  KETINEEAAEWDRVHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLN
  KIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAA
  TLEEMMTACQGVGGPGHKARVL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001802
• ORF Size: 696 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001802.1, NP_579880
• RefSeq ORF: 696
• MW: 25.7 kDa