11 kDa protein (NC_000883) Virus Tagged ORF Clone

CAT#: VC102083

Myc-DDK-tagged ORF clone of viral ORF for 11 kDa protein [Human parvovirus B19], codon optimized for human cell expression, YP_004928149

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 11 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102083 represents NCBI reference of YP_004928149 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAATAATACTACCGACATGGACATGAAAAGCCTCAAGAATTGCGGTCAGCCAAAAGCTGTGTGCA
  CCCACTGTAAACATTCCCCTCCTTGTCCCCAGCCAGGATGCGTCACGAAAAGACCTCCTGTGCCTCCCAG
  ACTTTACCTCCCACCTCCCATCCCCATCAGGCAGCCCAACACTAAGGACATTGACAATGTTGAGTTTAAG
  TACTTGACTCGCTATGAGCAACACGTCATCCGGATGTTGCGCCTCTGTAATATGTACCAGAACCTGGAGA
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102083 representing YP_004928149
  Red=Cloning sites Green=Tags
  
  MQNNTTDMDMKSLKNCGQPKAVCTHCKHSPPCPQPGCVTKRPPVPPRLYLPPPIPIRQPNTKDIDNVEFK
  YLTRYEQHVIRMLRLCNMYQNLEK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000883
• ORF Size: 282 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000883.2, YP_004928149
• RefSeq ORF: 282
• MW: 10.9 kDa