7.5 kDa protein (NC_000883) Virus Tagged ORF Clone

CAT#: VC102084

Myc-DDK-tagged ORF clone of viral ORF for 75 kDa protein [Human parvovirus B19], codon optimized for human cell expression, YP_004928145

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 7.5 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102084 represents NCBI reference of YP_004928145 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATGCCATCTACACAGACCAGTAAGCCCCCACAGCTTTCTCAGACCCCAGTTTCTGCAGCCGTAG
  TAGTGAAAGCCCTCAAGAATTCAGTGAAAGCCGCTTTCCTCACATCCAGCCCACAGGCGCCTGGGACACT
  CAAGCCAAGAGCCCTGGTGAGGCCTTCTCCCGGACCAGTTCAGGAAAACCATCTCTCTGAAGCGCAGTTT
  CCGCCAAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102084 representing YP_004928145
  Red=Cloning sites Green=Tags
  
  MQMPSTQTSKPPQLSQTPVSAAVVVKALKNSVKAAFLTSSPQAPGTLKPRALVRPSPGPVQENHLSEAQF
  PPKL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_000883
• ORF Size: 222 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_000883.2, YP_004928145
• RefSeq ORF: 222
• Locus ID: 11293634
• MW: 7.8 kDa