putative ankyrin repeat protein (NC_003494) Virus Tagged ORF Clone

CAT#: VC102415

Myc-DDK-tagged ORF clone of viral ORF for putative ankyrin repeat protein [Infectious spleen and kidney necrosis virus], codon optimized for human cell expression, NP_612347

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: putative ankyrin repeat protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102415 represents NCBI reference of NP_612347 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCGAAGAGCTGGCCGGGGCGCTGCGCACTGGCGGAAGTAATGGGCAGAGTATCCTTTTCGATG
  CTATTCGGTCAGGCACCATCACGGCCTTTACAGGGGTTCACGCCGATATCGTGAACACGGTGCGCGAGCA
  CAGCACCGGGAACACACTCCTCATGGCAGCGGTGATGACACGAGATTTGCTGATTATTAAACACGTGGTG
  GAAAACTTGGGATATAATACCTTCATGGGGATGAGGCTGTCAGATCATGCTACGGCGTTGCACCTCGTCG
  CTTGCCTGGCGGATCCTTATCATGGGTGTGTGAGGTATCTTCTGACTCAGTATGGGGCTCAAATGGCACC
  TGCCTTGACTATGACCACAAACGAAGATGACATGAATCCTCTGCATTACGCCTGCAAATATGGAGGAGTC
  CAGACAATGGTCCTTCTGGCAACAGTGATGAGCCAGTACGACTCATTTGCACAGGCTTGCTTCGCACTCA
  ACGCCAGCCTTGCACAGCCTGCCCTTCTTGCCCTGCATTATGACGAGCTGGGGGGAGCACAAAAGGCTTT
  TATTCTGGATAGCGTTGCCCCCTTGCAGGAGCGGTGGAACGGCCACAACGTTGCGCGGCTGATTAGAGGC
  GATCACAATCTTACTTGGTTTCTGATGGCACGGAATAACAAGGACTTCTTCGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102415 representing NP_612347
  Red=Cloning sites Green=Tags
  
  MLPEELAGALRTGGSNGQSILFDAIRSGTITAFTGVHADIVNTVREHSTGNTLLMAAVMTRDLLIIKHVV
  ENLGYNTFMGMRLSDHATALHLVACLADPYHGCVRYLLTQYGAQMAPALTMTTNEDDMNPLHYACKYGGV
  QTMVLLATVMSQYDSFAQACFALNASLAQPALLALHYDELGGAQKAFILDSVAPLQERWNGHNVARLIRG
  DHNLTWFLMARNNKDFFA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003494
• ORF Size: 684 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003494.1, NP_612347
• RefSeq ORF: 684
• Locus ID: 935349
• MW: 24.9 kDa