NS2 (NC_004906) Virus Tagged ORF Clone

CAT#: VC102435

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein 2 [Influenza A virus (A/Hong Kong/1073/99(H9N2))], codon optimized for human cell expression, YP_581750

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102435 represents NCBI reference of YP_581750 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCTAACACGGTTTCCTCTTTCCAGGACATACTTACCAGAATGTCCAAAATGCAACTCGGCTCAT
  CATCTGAGGACCTCAACGGTATGATTACCCAGTTCGAGAGTTTGAAATTGTACCGCGATTCTCTGGGCGA
  AGCGGTGATGCGGATGGGTGATTTGCATAGCCTCCAAAATAGAAACGGGAAATGGCGCGAACAGTTGAGC
  CAAAAGTTTGAAGAAATCAGATGGCTCATCGAGGAGATGAGACATAGACTGAGGATTACCGAGAACTCCT
  TTGAACAAATAACCTTTATGCAGGCTCTCCAGCTGTTGCTGGAAGTGGAACAGGAGATAAGAACTTTTTC
  TTTCCAGCTGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102435 representing YP_581750
  Red=Cloning sites Green=Tags
  
  MDSNTVSSFQDILTRMSKMQLGSSSEDLNGMITQFESLKLYRDSLGEAVMRMGDLHSLQNRNGKWREQLS
  QKFEEIRWLIEEMRHRLRITENSFEQITFMQALQLLLEVEQEIRTFSFQLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004906
• ORF Size: 363 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004906.1, YP_581750
• RefSeq ORF: 363
• Locus ID: 4036374
• MW: 14.4 kDa