PB1-F2 (NC_007372) Virus Tagged ORF Clone

CAT#: VC102466

Myc-DDK-tagged ORF clone of viral ORF for PB1-F2 protein [Influenza A virus (A/New York/392/2004(H3N2))], codon optimized for human cell expression, YP_308848

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PB1-F2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102466 represents NCBI reference of YP_308848 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGGAGCAGGACACTCCTTGGACCCAGTCCACTGAGCATACTAACATCCAGAGGCGGGGAAGTG
  GAAGGCAGATCCAGAAGCTTGGCCACCCCAATTCTACTCAGTTGATGGATCATTATCTCCGCATCATGAG
  CCAAGTCGATATGCACAAACAAACCGTGTCTTGGAGACTCTGGCCCAGCCTGAAAAATCCAACCCAGGTC
  TCTCTTAGAACACACGCACTTAAGCAATGGAAATCATTCAACAAACAGGGATGGACAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102466 representing YP_308848
  Red=Cloning sites Green=Tags
  
  MEQEQDTPWTQSTEHTNIQRRGSGRQIQKLGHPNSTQLMDHYLRIMSQVDMHKQTVSWRLWPSLKNPTQV
  SLRTHALKQWKSFNKQGWTN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007372
• ORF Size: 270 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007372.1, YP_308848
• RefSeq ORF: 270
• Locus ID: 3655160
• MW: 10.7 kDa