NS2 (NC_006306) Virus Tagged ORF Clone

CAT#: VC102498

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein 2 [Influenza C virus (C/Ann Arbor/1/50)], codon optimized for human cell expression, YP_002302328

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102498 represents NCBI reference of YP_002302328 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACAAGACTGTGAAGTCTACTAATCTCATGGCATTCGTAGCTACAAAAATGCTGGAACGGCAGG
  AGGATTTGGACACCTGTACAGAAATGCAGGTGGAGAAAATGAAAACTAGCACTAAGGCCAGGCTCAAAAC
  GGAGAGTTCCTTCGCACCCAGAACTTGGGAGGACGCCATCAAGGACGAAATCCTGAGACGGAGCGTTGAT
  ACCTCATCACTGGACAAATGGCCTGAGTTGAAGCAGGAACTGGAGAATGTGAGTGATGCCTTGAAAGCTG
  ATTCACTGTGGCTCCCCATGAAAAGTCTCAGTTTGTATTCCAAGGTGAGTAATCAAGAACCCTCCTCAAT
  CCCAATTGGCGAGATGAAGCATCAGATACTCACCCGACTGAAGCTCATTTGTTCCAGACTGGAGAAATTG
  GACCTGAATCTGTCCAAGGCAGTCCTGGGAATTCAAAACAGCGAAGACTTGATCCTGATCATCTACAATA
  GAGATGTGTGTAAGAACACCATTCTGATGATCAAGTCTCTGTGTAACTCCCTGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102498 representing YP_002302328
  Red=Cloning sites Green=Tags
  
  MSDKTVKSTNLMAFVATKMLERQEDLDTCTEMQVEKMKTSTKARLKTESSFAPRTWEDAIKDEILRRSVD
  TSSLDKWPELKQELENVSDALKADSLWLPMKSLSLYSKVSNQEPSSIPIGEMKHQILTRLKLICSRLEKL
  DLNLSKAVLGIQNSEDLILIIYNRDVCKNTILMIKSLCNSLI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006306
• ORF Size: 546 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006306.2, YP_002302328
• RefSeq ORF: 546
• Locus ID: 7011833
• MW: 20.8 kDa