envelope protein (NC_001563) Virus Tagged ORF Clone

CAT#: VC102506

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, NP_776014

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: envelope protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102506 represents NCBI reference of NP_776014 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAATTGTCTCGGGATGAGTAACAGAGACTTCTTGGAGGGGGTGAGCGGCGCAACCTGGGTGGATC
  TGGTACTCGAGGGGGACTCCTGCGTCACCATTATGTCAAAAGACAAGCCCACCATCGACGTGAAGATGAT
  GAACATGGAGGCCGCGAATCTCGCCGATGTGCGCAGTTATTGCTACCTGGCCTCAGTCTCAGATCTGTCC
  ACAAGAGCTGCCTGTCCTACAATGGGTGAGGCCCACAACGAGAAACGCGCCGACCCTGCATTTGTGTGTA
  AGCAAGGCGTGGTTGATAGGGGATGGGGTAATGGTTGCGGCCTCTTTGGAAAGGGGTCCATAGATACATG
  CGCCAAGTTTGCTTGCACAACTAAAGCGACCGGATGGATCATTCAAAAGGAGAACATAAAGTACGAAGTA
  GCAATATTTGTGCACGGTCCTACTACAGTGGAGTCTCACGGCAAAATCGGTGCAACGCAGGCCGGGAGGT
  TCAGTATCACTCCCAGCGCTCCTAGTTATACACTGAAGCTGGGCGAATACGGAGAGGTGACTGTCGATTG
  TGAGCCTCGCAGCGGGATCGACACTTCTGCATATTATGTCATGTCAGTCGGCGAGAAGAGCTTCCTCGTT
  CACAGAGAATGGTTCATGGATCTGAATCTGCCGTGGTCCTCAGCCGGCAGCACAACATGGAGGAACCGCG
  AGACTCTCATGGAGTTCGAAGAACCACACGCCACGAAACAGTCTGTCGTGGCGCTTGGGTCACAGGAGGG
  AGCCCTGCACCAGGCTCTGGCGGGGGCCATACCAGTGGAATTCAGCTCTAACACTGTGAAGCTGACCAGT
  GGACACCTGAAGTGTCGCGTTAAGATGGAGAAGCTCCAGCTGAAGGGTACCACTTACGGTGTATGCTCTA
  AAGCATTCAAGTTTGCAAGGACACCCGCCGACACAGGGCATGGCACCGTGGTGCTGGAACTGCAGTACAC
  TGGAACAGACGGCCCATGTAAAGTGCCTATTTCTTCTGTGGCCAGTCTGAATGACCTGACTCCAGTGGGA
  CGCCTCGTAACCGTGAATCCATTTGTGAGCGTGGCTACTGCTAACTCCAAAGTGCTCATAGAACTGGAGC
  CCCCTTTTGGCGACAGTTACATCGTGGTCGGCAGAGGTGAGCAGCAGATCAACCATCACTGGCACAAGAG
  CGGCTCCTCTATCGGGAAGGCATTTACAACCACTCTGCGCGGAGCCCAACGACTCGCAGCGTTGGGAGAT
  ACGGCTTGGGATTTTGGTAGTGTGGGCGGGGTTTTCACCTCAGTGGGCAAAGCCATTCATCAGGTATTCG
  GCGGGGCCTTCCGCAGCCTCTTTGGAGGCATGAGCTGGATTACACAGGGGTTGCTGGGAGCACTGCTGCT
  GTGGATGGGGATTAACGCCCGCGATAGGTCTATAGCCATGACATTTCTGGCTGTGGGAGGGGTCCTGCTG
  TTCCTGTCTGTGAATGTGCACGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102506 representing NP_776014
  Red=Cloning sites Green=Tags
  
  MFNCLGMSNRDFLEGVSGATWVDLVLEGDSCVTIMSKDKPTIDVKMMNMEAANLADVRSYCYLASVSDLS
  TRAACPTMGEAHNEKRADPAFVCKQGVVDRGWGNGCGLFGKGSIDTCAKFACTTKATGWIIQKENIKYEV
  AIFVHGPTTVESHGKIGATQAGRFSITPSAPSYTLKLGEYGEVTVDCEPRSGIDTSAYYVMSVGEKSFLV
  HREWFMDLNLPWSSAGSTTWRNRETLMEFEEPHATKQSVVALGSQEGALHQALAGAIPVEFSSNTVKLTS
  GHLKCRVKMEKLQLKGTTYGVCSKAFKFARTPADTGHGTVVLELQYTGTDGPCKVPISSVASLNDLTPVG
  RLVTVNPFVSVATANSKVLIELEPPFGDSYIVVGRGEQQINHHWHKSGSSIGKAFTTTLRGAQRLAALGD
  TAWDFGSVGGVFTSVGKAIHQVFGGAFRSLFGGMSWITQGLLGALLLWMGINARDRSIAMTFLAVGGVLL
  FLSVNVHA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001563
• ORF Size: 1494 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001563.2, NP_776014
• RefSeq ORF: 1494
• MW: 53.5 kDa