non-structural protein NS2b (NC_001563) Virus Tagged ORF Clone

CAT#: VC102509

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, NP_776017

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: non-structural protein NS2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102509 represents NCBI reference of NP_776017 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGGCCCGCGACTGAAGTGATGACTGCCGTGGGACTGATGTTTGCCATCGTGGGAGGACTGGCGG
  AGCTGGATATCGATAGTATGGCGATCCCCATGACAATTGCCGGCCTTATGTTTGCCGCCTTCGTGATCAG
  CGGCAAATCTACTGATATGTGGATCGAGCGAACAGCTGACATTACTTGGGAGTCCGACGCCGAGATAACA
  GGGAGCAGCGAGCGGGTTGATGTAAGGCTGGACGACGACGGCAATTTTCAGCTCATGAACGATCCTGGTG
  CTCCCTGGAAAATTTGGATGCTCCGCATGGCATGTCTGGCTATCTCAGCATACACACCCTGGGCTATTTT
  GCCCTCCGTTATCGGTTTTTGGATAACCTTGCAATACACCAAGCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102509 representing NP_776017
  Red=Cloning sites Green=Tags
  
  MGWPATEVMTAVGLMFAIVGGLAELDIDSMAIPMTIAGLMFAAFVISGKSTDMWIERTADITWESDAEIT
  GSSERVDVRLDDDGNFQLMNDPGAPWKIWMLRMACLAISAYTPWAILPSVIGFWITLQYTKR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001563
• ORF Size: 396 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001563.2, NP_776017
• RefSeq ORF: 396
• MW: 14.6 kDa