non-structural protein NS3 (NC_001563) Virus Tagged ORF Clone

CAT#: VC102510

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, NP_776018

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: non-structural protein NS3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102510 represents NCBI reference of NP_776018 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGGCGTTCTTTGGGACACCCCCAGCCCTAAAGAATACAAAAAGGGGGACACAACTACCGGCGTCT
  ACAGGATAATGACCCGGGGCCTTCTGGGATCCTATCAAGCCGGAGCCGGCGTCATGGTTGAGGGGGTATT
  TCACACACTCTGGCATACTACCAAGGGCGCAGCCCTGATGAGCGGCGAAGGCAGGCTGGATCCGTATTGG
  GGTTCCGTGAAGGAGGATCGACTCTGCTATGGCGGTCCATGGAAGCTTCAACATAAATGGAACGGTCATG
  ACGAGGTGCAGATGATCGTGGTGGAGCCTGGAAAGAACGTGAAGAACGTCCAGACTAAACCTGGGGTATT
  TAAAACTCCTGAAGGTGAGATCGGCGCCGTTACTCTGGATTATCCGACAGGCACTAGCGGCTCACCCATA
  GTTGACAAGAACGGGGACGTGATAGGCCTGTATGGGAATGGGGTCATCATGCCCAATGGATCTTACATCA
  GCGCCATTGTCCAGGGCGAGAGAATGGAAGAGCCCGCTCCAGCTGGTTTCGAGCCAGAAATGCTGAGGAA
  GAAGCAGATTACAGTGCTGGATCTGCATCCCGGCGCAGGTAAGACTAGGAAAATACTGCCACAGATTATC
  AAGGAAGCCATTAACAAACGCCTGCGGACAGCTGTCCTGGCCCCCACCAGAGTGGTGGCAGCCGAGATGT
  CCGAGGCCCTTCGGGGACTCCCGATCAGGTACCAAACCTCAGCGGTCCATAGGGAGCATTCCGGCAATGA
  GATCGTGGATGTGATGTGCCATGCGACCCTGACGCACAGACTTATGAGTCCTCACCGGGTGCCTAATTAC
  AACCTGTTTATTATGGACGAGGCACACTTTACAGATCCAGCCAGTATCGCCGCCCGCGGCTACATCGCAA
  CAAAAGTGGAACTGGGTGAGGCTGCCGCCATATTTATGACCGCAACCCCCCCAGGAACATCCGATCCCTT
  CCCAGAGTCCAACGCCCCTATATCCGACATGCAGACCGAGATTCCAGACAGGGCTTGGAACACTGGGTAC
  GAGTGGATTACCGAATACGTTGGGAAGACAGTTTGGTTTGTGCCAAGCGTAAAGATGGGCAATGAGATAG
  CTCTCTGCCTCCAACGCGCAGGCAAAAAAGTCATTCAGCTGAATAGGAAAAGTTACGAAACAGAATATCC
  CAAGTGCAAGAATGATGACTGGGACTTCGTAATTACCACAGACATTAGCGAGATGGGAGCTAATTTTAAA
  GCTAGCAGGGTGATCGACAGTCGCAAGTCTGTTAAACCTACGATCATTGAAGAAGGGGATGGCAGGGTTA
  TCCTTGGTGAGCCTTCCGCCATTACAGCGGCCTCCGCCGCCCAACGGAGAGGGAGAATCGGCCGGAACCC
  ATCCCAAGTTGGAGACGAATACTGTTACGGCGGGCACACAAACGAAGACGACTCCAACTTTGCCCATTGG
  ACCGAAGCCCGGATAATGCTCGATAATATCAACATGCCCAATGGGTTGGTCGCCCAGCTGTATCAGCCCG
  AGAGAGAAAAAGTATATACCATGGACGGGGAGTATCGGCTTCGCGGCGAGGAACGGAAGAACTTCTTGGA
  GTTCCTCAGAACTGCCGACCTGCCCGTGTGGCTGGCATATAAAGTGGCGGCAGCCGGCATTTCATACCAC
  GACCGAAAGTGGTGCTTTGACGGGCCAAGGACCAACACCATCCTGGAGGACAACAACGAAGTGGAAGTGA
  TAACAAAGCTGGGAGAGCGGAAGATTTTGCGCCCTCGGTGGGCCGATGCCCGAGTGTACTCAGATCACCA
  GGCCCTGAAATCCTTTAAGGACTTTGCAAGTGGAAAGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102510 representing NP_776018
  Red=Cloning sites Green=Tags
  
  MGGVLWDTPSPKEYKKGDTTTGVYRIMTRGLLGSYQAGAGVMVEGVFHTLWHTTKGAALMSGEGRLDPYW
  GSVKEDRLCYGGPWKLQHKWNGHDEVQMIVVEPGKNVKNVQTKPGVFKTPEGEIGAVTLDYPTGTSGSPI
  VDKNGDVIGLYGNGVIMPNGSYISAIVQGERMEEPAPAGFEPEMLRKKQITVLDLHPGAGKTRKILPQII
  KEAINKRLRTAVLAPTRVVAAEMSEALRGLPIRYQTSAVHREHSGNEIVDVMCHATLTHRLMSPHRVPNY
  NLFIMDEAHFTDPASIAARGYIATKVELGEAAAIFMTATPPGTSDPFPESNAPISDMQTEIPDRAWNTGY
  EWITEYVGKTVWFVPSVKMGNEIALCLQRAGKKVIQLNRKSYETEYPKCKNDDWDFVITTDISEMGANFK
  ASRVIDSRKSVKPTIIEEGDGRVILGEPSAITAASAAQRRGRIGRNPSQVGDEYCYGGHTNEDDSNFAHW
  TEARIMLDNINMPNGLVAQLYQPEREKVYTMDGEYRLRGEERKNFLEFLRTADLPVWLAYKVAAAGISYH
  DRKWCFDGPRTNTILEDNNEVEVITKLGERKILRPRWADARVYSDHQALKSFKDFASGKR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001563
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001563.2, NP_776018
• RefSeq ORF: 1860
• MW: 69.1 kDa