nonstructural protein 2B (NC_009942) Virus Tagged ORF Clone

CAT#: VC102521

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, YP_001527883

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein 2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102521 represents NCBI reference of YP_001527883 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGGCCAGCTACGGAGGTGATGACAGCCGTTGGTCTCATGTTCGCTATCGTTGGCGGGCTCGCTG
  AACTGGATATCGATAGCATGGCGATACCAATGACGATTGCGGGACTGATGTTCGCTGCCTTTGTTATATC
  CGGGAAATCCACCGACATGTGGATAGAACGAACAGCTGACATCTCTTGGGAGTCAGATGCAGAAATCACA
  GGCTCCAGTGAAAGGGTCGACGTGAGGCTCGACGATGATGGCAATTTTCAACTTATGAACGACCCCGGCG
  CTCCCTGGAAGATATGGATGCTGAGGATGGTCTGTCTCGCCATCAGTGCCTACACTCCATGGGCCATCCT
  TCCATCAGTGGTGGGTTTCTGGATCACTCTTCAGTACACCAAGCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102521 representing YP_001527883
  Red=Cloning sites Green=Tags
  
  MGWPATEVMTAVGLMFAIVGGLAELDIDSMAIPMTIAGLMFAAFVISGKSTDMWIERTADISWESDAEIT
  GSSERVDVRLDDDGNFQLMNDPGAPWKIWMLRMVCLAISAYTPWAILPSVVGFWITLQYTKR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009942
• ORF Size: 396 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009942.1, YP_001527883
• RefSeq ORF: 396
• MW: 14.6 kDa