envelope protein E (NC_012532) Virus Tagged ORF Clone

CAT#: VC102530

Myc-DDK-tagged ORF clone of viral ORF for envelope protein E [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227198.1

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: envelope protein E
• Synonyms: ZIKV_gp1, ZIKV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102530 represents NCBI reference of YP_009227198 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAGGTGTATAGGGGTATCTAACCGCGATTTTGTCGAAGGTATGTCCGGCGGCACCTGGGTAGACG
  TGGTGCTCGAGCACGGGGGTTGTGTGACTGTCATGGCGCAGGATAAGCCTACTGTGGACATAGAGCTGGT
  GACCACTACAGTTTCTAATATGGCCGAAGTGAGAAGTTACTGTTACGAGGCCTCCATTTCTGACATGGCT
  AGCGACTCCAGGTGCCCGACTCAGGGCGAGGCCTATCTTGATAAACAAAGTGACACTCAATATGTGTGCA
  AAAGAACTCTCGTCGACCGAGGCTGGGGTAATGGTTGTGGTCTGTTCGGCAAAGGGAGTCTCGTCACATG
  CGCTAAGTTTACTTGTAGTAAGAAAATGACCGGCAAAAGCATCCAGCCAGAAAACTTGGAGTACAGGATT
  ATGCTCTCCGTTCATGGTTCCCAGCACAGCGGTATGATCGGCTACGAGACGGATGAGGATCGGGCTAAAG
  TCGAGGTGACCCCAAATTCCCCCAGAGCTGAAGCGACCCTTGGCGGCTTCGGATCACTTGGACTGGACTG
  CGAGCCGCGAACAGGCCTTGATTTCTCTGACCTGTATTACCTGACAATGAACAATAAGCATTGGCTCGTA
  CACAAAGAATGGTTTCATGACATACCACTGCCTTGGCACGCAGGAGCCGACACCGGGACACCCCATTGGA
  ATAATAAAGAAGCATTGGTGGAATTTAAAGATGCACACGCGAAGAGACAGACTGTGGTAGTGCTGGGTTC
  CCAGGAAGGCGCGGTGCATACAGCCTTGGCAGGCGCACTGGAAGCCGAGATGGACGGCGCGAAAGGAAGA
  CTTTTTAGCGGACATCTGAAATGTAGGCTGAAGATGGATAAATTGCGACTGAAAGGGGTTTCTTATTCTC
  TCTGTACGGCCGCATTTACATTTACTAAAGTCCCAGCAGAGACATTGCACGGCACAGTCACTGTGGAAGT
  CCAATACGCAGGAACCGATGGACCATGCAAAATTCCGGTTCAGATGGCCGTAGATATGCAGACATTGACA
  CCAGTGGGCAGGCTTATTACCGCCAATCCCGTTATTACTGAAAGCACCGAGAACTCTAAGATGATGCTCG
  AACTGGACCCCCCCTTTGGCGACTCATATATTGTCATTGGCGTGGGCGATAAGAAAATCACACATCACTG
  GCATCGCTCCGGCAGCACAATTGGTAAGGCCTTTGAAGCCACTGTCAGGGGAGCAAAGAGAATGGCTGTT
  CTGGGTGATACTGCTTGGGATTTCGGATCTGTGGGAGGTGTGTTCAATAGTTTGGGGAAAGGAATCCACC
  AGATCTTCGGAGCTGCCTTTAAAAGTTTGTTCGGAGGGATGTCCTGGTTTTCTCAGATCTTGATCGGTAC
  ACTCCTGGTTTGGCTCGGCCTCAACACTAAAAACGGATCTATTTCTCTGACATGCCTTGCTCTTGGAGGC
  GTTATGATCTTTTTGTCAACAGCAGTATCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102530 representing YP_009227198
  Red=Cloning sites Green=Tags
  
  MIRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMA
  SDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRI
  MLSVHGSQHSGMIGYETDEDRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLV
  HKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGR
  LFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPAETLHGTVTVEVQYAGTDGPCKIPVQMAVDMQTLT
  PVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKRMAV
  LGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKNGSISLTCLALGG
  VMIFLSTAVSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012532
• ORF Size: 1503 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_012532.1, YP_009227198
• RefSeq ORF: 1503 bp
• MW: 54.2 kDa