nonstructural protein NS4B (NC_012532) Virus Tagged ORF Clone

CAT#: VC102537

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4B [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227204.1

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein NS4B
• Synonyms: ZIKV_gp1, ZIKV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102537 represents NCBI reference of YP_009227204 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGAACTTGGTTGGCTGGAACGAACAAAAAACGATATTGCTCACCTGATGGGACGACGAGAAGAGG
  GTGCTACGATGGGATTTTCTATGGATATTGACCTCCGACCTGCCAGCGCCTGGGCAATCTATGCTGCCTT
  GACTACTCTGATTACTCCCGCAGTGCAACACGCAGTTACCACATCTTACAATAACTATTCTCTCATGGCC
  ATGGCCACACAGGCTGGGGTGCTGTTCGGTATGGGTAAAGGAATGCCATTTATGCACGGCGATCTCGGTG
  TACCCCTCTTGATGATGGGCTGCTATTCTCAACTGACCCCTCTGACCCTGATCGTAGCTATTATACTGCT
  GGTCGCACATTATATGTACCTCATCCCAGGTCTTCAGGCAGCGGCAGCGCGGGCAGCACAGAAGAGGACC
  GCTGCCGGAATCATGAAGAACCCAGTAGTGGATGGAATCGTCGTCACAGACATAGACACCATGACTATTG
  ATCCTCAGGTGGAAAAGAAAATGGGACAGGTCTTGCTCATTGCAGTTGCTATTTCTTCAGCCGTCTTGCT
  TAGAACAGCTTGGGGGTGGGGAGAGGCCGGAGCTTTGATCACAGCCGCCACTTCAACACTCTGGGAAGGC
  TCACCTAACAAATATTGGAACTCATCTACAGCGACGTCTCTTTGTAACATTTTCAGAGGGTCTTATCTCG
  CGGGCGCATCACTCATATACACTGTCACAAGGAATGCGGGCCTGGTAAAGCGGCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102537 representing YP_009227204
  Red=Cloning sites Green=Tags
  
  MNELGWLERTKNDIAHLMGRREEGATMGFSMDIDLRPASAWAIYAALTTLITPAVQHAVTTSYNNYSLMA
  MATQAGVLFGMGKGMPFMHGDLGVPLLMMGCYSQLTPLTLIVAIILLVAHYMYLIPGLQAAAARAAQKRT
  AAGIMKNPVVDGIVVTDIDTMTIDPQVEKKMGQVLLIAVAISSAVLLRTAWGWGEAGALITAATSTLWEG
  SPNKYWNSSTATSLCNIFRGSYLAGASLIYTVTRNAGLVKRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012532
• ORF Size: 756 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_012532.1, YP_009227204
• RefSeq ORF: 756 bp
• MW: 27.1 kDa