membrane glycoprotein M (NC_012532) Virus Tagged ORF Clone

CAT#: VC102539

Myc-DDK-tagged ORF clone of viral ORF for Membrane glycoprotein M [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227208.1

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: membrane glycoprotein M
• Synonyms: ZIKV_gp1, ZIKV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102539 represents NCBI reference of YP_009227208 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTTACACTCCCGTCCCACTCTACTCGAAAACTTCAGACGCGCTCTCAAACATGGCTCGAATCCC
  GCGAATACACCAAACATCTCATCAAGGTTGAAAATTGGATCTTCAGAAACCCCGGATTTGCTCTTGTCGC
  TGTGGCAATTGCTTGGCTCCTCGGCTCCTCCACATCCCAGAAAGTGATCTATCTTGTTATGATCTTGCTC
  ATCGCCCCCGCATATTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102539 representing YP_009227208
  Red=Cloning sites Green=Tags
  
  MAVTLPSHSTRKLQTRSQTWLESREYTKHLIKVENWIFRNPGFALVAVAIAWLLGSSTSQKVIYLVMILL
  IAPAYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012532
• ORF Size: 228 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012532.1, YP_009227208
• RefSeq ORF: 228
• MW: 8.6 kDa