protein pr (NC_012532) Virus Tagged ORF Clone

CAT#: VC102540

Myc-DDK-tagged ORF clone of viral ORF for Protein pr [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227207.1

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: protein pr
• Synonyms: ZIKV_gp1, ZIKV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102540 represents NCBI reference of YP_009227207 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAATTACAAGAAGGGGTTCAGCATACTATATGTATCTCGATCGAAGTGACGCGGGAAAAGCCA
  TAAGTTTTGCAACTACACTCGGGGTAAATAAATGTCATGTTCAAATTATGGATCTTGGGCACATGTGTGA
  CGCGACAATGAGTTATGAATGTCCAATGCTTGATGAGGGCGTAGAGCCAGACGACGTGGATTGCTGGTGC
  AATACGACATCCACATGGGTTGTATACGGAACCTGTCATCACAAAAAGGGCGAGGCTAGAAGGAGTAGAA
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102540 representing YP_009227207
  Red=Cloning sites Green=Tags
  
  MAEITRRGSAYYMYLDRSDAGKAISFATTLGVNKCHVQIMDLGHMCDATMSYECPMLDEGVEPDDVDCWC
  NTTSTWVVYGTCHHKKGEARRSRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012532
• ORF Size: 282 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012532.1, YP_009227207
• RefSeq ORF: 282
• MW: 10.6 kDa