nonstructural protein NS1 (NC_001474) Virus Tagged ORF Clone

CAT#: VC102546

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS1 [Dengue virus 2], codon optimized for human cell expression, NP_739584

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein NS1
• Synonyms: DENV_gp1, Dengue, DENV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102546 represents NCBI reference of NP_739584 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCGGATGTGTCGTGTCCTGGAAGAACAAGGAACTGAAATGCGGCTCTGGTATATTCATTACAG
  ACAATGTGCATACTTGGACCGAGCAATATAAATTCCAACCCGAATCCCCATCCAAATTGGCAAGTGCAAT
  TCAGAAAGCCCATGAAGAGGGAATCTGCGGAATTCGCTCAGTAACAAGACTCGAAAATCTTATGTGGAAG
  CAAATAACCCCAGAACTTAATCACATACTGTCAGAAAACGAAGTTAAACTCACTATCATGACCGGCGACA
  TCAAAGGAATTATGCAAGCTGGTAAAAGGAGTCTTAGACCTCAGCCTACGGAATTGAAATATAGTTGGAA
  AACATGGGGGAAAGCAAAAATGTTGTCTACCGAATCACATAACCAAACGTTCCTGATTGATGGACCTGAG
  ACCGCAGAGTGCCCAAATACAAATCGAGCGTGGAATTCACTGGAGGTTGAGGACTATGGGTTCGGTGTCT
  TTACAACCAACATTTGGCTCAAGCTCAAAGAAAAACAGGATGTGTTCTGTGACTCCAAACTTATGTCTGC
  CGCGATCAAAGATAACCGAGCTGTGCATGCTGACATGGGATATTGGATTGAATCAGCCCTGAATGATACC
  TGGAAAATTGAGAAGGCTAGTTTTATAGAGGTGAAGAATTGCCATTGGCCTAAGAGTCATACACTGTGGT
  CTAACGGCGTGCTGGAATCCGAAATGATAATCCCGAAGAACCTTGCAGGACCTGTCTCCCAACATAACTA
  TCGCCCAGGCTATCATACCCAAATTACTGGGCCCTGGCACCTTGGAAAACTGGAAATGGATTTCGACTTC
  TGCGACGGAACTACAGTGGTCGTGACCGAAGACTGCGGCAACAGAGGACCATCACTGCGCACGACAACAG
  CCAGCGGAAAACTCATCACAGAGTGGTGCTGCCGATCTTGTACACTCCCCCCCCTTCGGTACAGGGGCGA
  GGATGGGTGCTGGTACGGGATGGAAATCCGACCTTTGAAAGAAAAAGAAGAAAATTTGGTGAATAGTCTG
  GTCACAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102546 representing NP_739584
  Red=Cloning sites Green=Tags
  
  MDSGCVVSWKNKELKCGSGIFITDNVHTWTEQYKFQPESPSKLASAIQKAHEEGICGIRSVTRLENLMWK
  QITPELNHILSENEVKLTIMTGDIKGIMQAGKRSLRPQPTELKYSWKTWGKAKMLSTESHNQTFLIDGPE
  TAECPNTNRAWNSLEVEDYGFGVFTTNIWLKLKEKQDVFCDSKLMSAAIKDNRAVHADMGYWIESALNDT
  WKIEKASFIEVKNCHWPKSHTLWSNGVLESEMIIPKNLAGPVSQHNYRPGYHTQITGPWHLGKLEMDFDF
  CDGTTVVVTEDCGNRGPSLRTTTASGKLITEWCCRSCTLPPLRYRGEDGCWYGMEIRPLKEKEENLVNSL
  VTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001474
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001474.2, NP_739584
• RefSeq ORF: 1059
• MW: 40 kDa