Human alpha 1 Fetoprotein (AFP) activation kit by CRISPRa

CAT#: GA100122

AFP CRISPRa kit - CRISPR gene activation of human alpha fetoprotein

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: AFP
• Locus ID: 174
• Kit Components:

  GA100122G1, alpha 1 Fetoprotein gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100122G2, alpha 1 Fetoprotein gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100122G3, alpha 1 Fetoprotein gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001134, NM_001354717
• Synonyms: AFPD; FETA; HPAFP
• Summary: 'This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019]'