Human ATP2B4 activation kit by CRISPRa

CAT#: GA100348

ATP2B4 CRISPRa kit - CRISPR gene activation of human ATPase plasma membrane Ca2+ transporting 4

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ATP2B4
• Locus ID: 493
• Kit Components:

  GA100348G1, ATP2B4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100348G2, ATP2B4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100348G3, ATP2B4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001001396, NM_001684, NM_001365783, NM_001365784
• Synonyms: ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x
• Summary: 'The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'