Human CAD activation kit by CRISPRa

CAT#: GA100553

CAD CRISPRa kit - CRISPR gene activation of human carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CAD
• Locus ID: 790
• Kit Components:

  GA100553G1, CAD gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100553G2, CAD gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100553G3, CAD gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001306079, NM_004341
• Synonyms: CAD trifunctional protein; carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase; carbamoylphosphate synthetase 2/aspartate transcarbamylase/dihydroorotase; multifunctional protein CAD
• Summary: 'The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]'