Human CHRNB4 activation kit by CRISPRa

CAT#: GA100820

CHRNB4 CRISPRa kit - CRISPR gene activation of human cholinergic receptor nicotinic beta 4 subunit

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CHRNB4
• Locus ID: 1143
• Kit Components:

  GA100820G1, CHRNB4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100820G2, CHRNB4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100820G3, CHRNB4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000750, NM_001256567
• Synonyms: cholinergic receptor, nicotinic, beta 4; cholinergic receptor, nicotinic, beta polypeptide 4; neuronal nicotinic receptor beta 4 subunit
• Summary: 'This gene is found within a conserved gene cluster and encodes one of the beta subunits of the nicotinic acetylcholine receptor (nAChRs) superfamily which form ligand-gated ion channels with a central pore that forms a cation channel. Neuronal nAChRs are pentameric structures that can be either homomeric or heteromeric, with heteromeric structures containing both alpha and beta subunits. Each subunit contains an extracellular amino terminus and four transmembrane domains. Nicotine is one of the agonists that binds to the receptor. Variants in this gene have been associated with nicotine dependence and lung cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]'