Human DCTN1 activation kit by CRISPRa

CAT#: GA101155

DCTN1 CRISPRa kit - CRISPR gene activation of human dynactin subunit 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DCTN1
• Locus ID: 1639
• Kit Components:

  GA101155G1, DCTN1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101155G2, DCTN1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101155G3, DCTN1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001135040, NM_001135041, NM_001190836, NM_001190837, NM_004082, NM_023019, NR_033935
• Synonyms: DAP-150; DP-150; P135
• Summary: 'This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]'