Human EWSR1 activation kit by CRISPRa

CAT#: GA101484

EWSR1 CRISPRa kit - CRISPR gene activation of human EWS RNA binding protein 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: EWSR1
• Locus ID: 2130
• Kit Components:

  GA101484G1, EWSR1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101484G2, EWSR1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101484G3, EWSR1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001163285, NM_001163286, NM_001163287, NM_005243, NM_013986
• Synonyms: bK984G1.4; EWS; EWS-FLI1
• Summary: 'This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]'