Human MBD1 activation kit by CRISPRa

CAT#: GA102830

MBD1 CRISPRa kit - CRISPR gene activation of human methyl-CpG binding domain protein 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: MBD1
• Locus ID: 4152
• Kit Components:

  GA102830G1, MBD1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102830G2, MBD1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102830G3, MBD1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001204136, NM_001204137, NM_001204138, NM_001204139, NM_001204140, NM_001204141, NM_001204142, NM_001204143, NM_001204151, NM_002384, NM_015844, NM_015845, NM_015846, NM_015847, NM_001323942, NM_001323947, NM_001323949, NM_001323950, NM_001323951, NM_001323952, NM_001323953, NM_001323954
• Synonyms: CXXC3; PCM1; RFT
• Summary: 'The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]'