Human NEDD4 activation kit by CRISPRa

CAT#: GA103159

NEDD4 CRISPRa kit - CRISPR gene activation of human NEDD4 E3 ubiquitin protein ligase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: NEDD4
• Locus ID: 4734
• Kit Components:

  GA103159G1, NEDD4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103159G2, NEDD4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103159G3, NEDD4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001284338, NM_001284339, NM_001284340, NM_006154, NM_198400, NR_104302, NM_001329212
• Synonyms: NEDD4-1; RPF1
• Summary: 'This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]'