Human PTCH1 activation kit by CRISPRa

CAT#: GA103893

PTCH1 CRISPRa kit - CRISPR gene activation of human patched 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PTCH1
• Locus ID: 5727
• Kit Components:

  GA103893G1, PTCH1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103893G2, PTCH1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103893G3, PTCH1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000264, NM_001083602, NM_001083603, NM_001083604, NM_001083605, NM_001083606, NM_001083607, NM_001354918, NM_001354919, NR_149061
• Synonyms: BCNS; HPE7; NBCCS; PTC; PTC1; PTCH; PTCH11
• Summary: 'This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]'