Human RAP1GAP activation kit by CRISPRa

CAT#: GA104019

RAP1GAP CRISPRa kit - CRISPR gene activation of human RAP1 GTPase activating protein

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: RAP1GAP
• Locus ID: 5909
• Kit Components:

  GA104019G1, RAP1GAP gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104019G2, RAP1GAP gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104019G3, RAP1GAP gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001145657, NM_001145658, NM_001330383, NM_002885, NM_001350524, NM_001350525, NM_001350526, NM_001350527, NM_001350528
• Synonyms: RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP
• Summary: 'This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Aug 2011]'