Human UBE2D1 activation kit by CRISPRa

CAT#: GA105041

UBE2D1 CRISPRa kit - CRISPR gene activation of human ubiquitin conjugating enzyme E2 D1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: UBE2D1
• Locus ID: 7321
• Kit Components:

  GA105041G1, UBE2D1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA105041G2, UBE2D1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA105041G3, UBE2D1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001204880, NM_003338
• Synonyms: E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A
• Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]