Human Wilms Tumor Protein (WT1) activation kit by CRISPRa

CAT#: GA105163

WT1 CRISPRa kit - CRISPR gene activation of human WT1 transcription factor

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: WT1
• Locus ID: 7490
• Kit Components:

  GA105163G1, Wilms Tumor Protein gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA105163G2, Wilms Tumor Protein gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA105163G3, Wilms Tumor Protein gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000378, NM_001198551, NM_001198552, NM_024424, NM_024425, NM_024426, NM_001367854, NR_160306
• Synonyms: AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33
• Summary: 'This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]'