Human DPM1 activation kit by CRISPRa

CAT#: GA105857

DPM1 CRISPRa kit - CRISPR gene activation of human dolichyl-phosphate mannosyltransferase subunit 1, catalytic

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DPM1
• Locus ID: 8813
• Kit Components:

  GA105857G1, DPM1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA105857G2, DPM1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA105857G3, DPM1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001317034, NM_001317035, NM_001317036, NM_003859, NR_133648
• Synonyms: CDGIE; MPDS
• Summary: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Mutations in this gene are associated with congenital disorder of glycosylation type Ie. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]