Human Pumilio 1 (PUM1) activation kit by CRISPRa

CAT#: GA106509

PUM1 CRISPRa kit - CRISPR gene activation of human pumilio RNA binding family member 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PUM1
• Locus ID: 9698
• Kit Components:

  GA106509G1, PUM1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA106509G2, PUM1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA106509G3, PUM1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001020658, NM_014676
• Synonyms: HSPUM; PUMH; PUMH1; PUML1
• Summary: This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]