Human ARFBP1 (HUWE1) activation kit by CRISPRa

CAT#: GA106828

HUWE1 CRISPRa kit - CRISPR gene activation of human HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: HUWE1
• Locus ID: 10075
• Kit Components:

  GA106828G1, HUWE1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA106828G2, HUWE1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA106828G3, HUWE1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_031407
• Synonyms: ARF-BP1; HECTH9; HSPC272; Ib772; LASU1; MULE; URE-B1; UREB1
• Summary: This gene encodes a protein containing a C-terminal HECT (E6AP type E3 ubiquitin protein ligase) domain that functions as an E3 ubiquitin ligase. The encoded protein is required for the ubiquitination and subsequent degradation of the anti-apoptotic protein Mcl1 (myeloid cell leukemia sequence 1 (BCL2-related)). This protein also ubiquitinates the p53 tumor suppressor, core histones, and DNA polymerase beta. Mutations in this gene are associated with Turner type X-linked syndromic cognitive disability. [provided by RefSeq, Aug 2013]