Mouse Ccnd3 activation kit by CRISPRa

CAT#: GA200582

Ccnd3 CRISPRa kit - CRISPR gene activation of mouse cyclin D3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Ccnd3
• Locus ID: 12445
• Kit Components:

  GA200582G1, Ccnd3 gRNA vector 1 in pCas-Guide-CRISPRa

  GA200582G2, Ccnd3 gRNA vector 2 in pCas-Guide-CRISPRa

  GA200582G3, Ccnd3 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001081635, NM_001081636, NM_007632
• Synonyms: 9230106B05Rik; AA682053; AL024085; AW146355; C78795
• Summary: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. [UniProtKB/Swiss-Prot Function]