Mouse Col2a1 activation kit by CRISPRa

CAT#: GA200812

Col2a1 CRISPRa kit - CRISPR gene activation of mouse collagen, type II, alpha 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Col2a1
• Locus ID: 12824
• Kit Components:

  GA200812G1, Col2a1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA200812G2, Col2a1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA200812G3, Col2a1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001113515, NM_031163
• Synonyms: Col2; Col2a; Col2a-1; Del1; Dmm; Lpk; Rgsc856
• Summary: This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Dec 2015]