Mouse Lfng activation kit by CRISPRa

CAT#: GA202441

Lfng CRISPRa kit - CRISPR gene activation of mouse LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Lfng
• Locus ID: 16848
• Kit Components:

  GA202441G1, Lfng gRNA vector 1 in pCas-Guide-CRISPRa

  GA202441G2, Lfng gRNA vector 2 in pCas-Guide-CRISPRa

  GA202441G3, Lfng gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_008494
• Synonyms: AW061165
• Summary: Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules inhibiting Jagged mediated Notch signaling. Essential mediator of somite segmentation and patterning. During somite boundary formation, it restricts Notch activity in the presomitic mesoderm to a boundary-formig territory in the posterior half of the prospective somite. In this region, Notch function activates a set of genes that are involved in boundary formation and in anterior-posterior somite identity. Ectopically expressed in the thymus, Lfgn inhibits Notch signaling which results in inhibition of T-cell commitment and promotes B-cell development in lymphoid progenitors. May play a role in boundary formation of the enamel knot. [UniProtKB/Swiss-Prot Function]