Mouse Elane activation kit by CRISPRa

CAT#: GA205330

Elane CRISPRa kit - CRISPR gene activation of mouse elastase, neutrophil expressed

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Elane
• Locus ID: 50701
• Kit Components:

  GA205330G1, Elane gRNA vector 1 in pCas-Guide-CRISPRa

  GA205330G2, Elane gRNA vector 2 in pCas-Guide-CRISPRa

  GA205330G3, Elane gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_015779
• Synonyms: Ela2; F430011M15Rik; NE
• Summary: This gene encodes a member of the chymotrypsin-like family of serine protease enzymes that hydrolyzes a broad range of protein substrates including elastin. This gene is expressed by neutrophils where the encoded enzyme is stored in azurophil granules. Upon neutrophil activation, the active enzyme is released into the extracellular mileu. Mice lacking the encoded protein exhibit increased susceptibility to sepsis and death following intraperitoneal infection with Gram negative bacteria. This gene is located adjacent to a related proteinase gene on chromosome 10. [provided by RefSeq, Jul 2016]