PTCH1 Human Gene Knockout Kit (CRISPR)

CAT#: KN411551

PTCH1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.

Product Images

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Specifications

Product Data

• Format: 2 gRNA vectors, 1 linear donor
• Donor DNA: EF1a-GFP-P2A-Puro
• Symbol: PTCH1
• Locus ID: 5727
• Disclaimer: The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000264, NM_001083602, NM_001083603, NM_001083604, NM_001083605, NM_001083606, NM_001083607, NM_001354918, NM_001354919, NR_149061
• Synonyms: BCNS; HPE7; NBCCS; PTC; PTC1; PTCH; PTCH11
• Summary: 'This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]'