NDUFB9 (NM_005005) Human Mass Spec Standard

CAT#: PH300223

NDUFB9 MS Standard C13 and N15-labeled recombinant protein (NP_004996)

Product Images

Coomassie blue staining of purified NDUFB9 protein (Cat# TP300223). The protein was produced from HEK293T cells transfected with NDUFB9 cDNA clone (Cat# RC200223) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200223
• Predicted MW: 21.8 kDa
• Protein Sequence:
  Sequence Link

  >RC200223 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFLASGPYLTHQQKVLRLYKRALRHLESWCVQRDKYRYFACLMRARFEEHKNEKDMAKATQLLKEAEEE
  FWYRQHPQPYIFPDSPGGTSYERYDCYKVPEWCLDDWHPSEKAMYPDYFAKREQWKKLRRESWEREVKQL
  QEETPPGGPLTEALPPARKEGDLPPLWWYIVTRPRERPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004996
• RefSeq Size: 736
• RefSeq ORF: 537
• Synonyms: B22; CI-B22; LYRM3; MC1DN24; UQOR22
• Locus ID: 4715
• UniProt ID: Q9Y6M9
• Cytogenetics: 8q24.13
• Summary: 'The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]'
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease