Prohibitin (PHB) (NM_002634) Human Mass Spec Standard

CAT#: PH301229

PHB MS Standard C13 and N15-labeled recombinant protein (NP_002625)

Product Images

Coomassie blue staining of purified PHB protein (Cat# TP301229). The protein was produced from HEK293T cells transfected with PHB cDNA clone (Cat# RC201229) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201229
• Predicted MW: 29.6 kDa
• Protein Sequence:
  Sequence Link

  >RC201229 representing NM_002634
  Red=Cloning site Green=Tags(s)
  
  MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCR
  SRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELI
  TQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAA
  IISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002625
• RefSeq Size: 1826
• RefSeq ORF: 816
• Synonyms: HEL-215; HEL-S-54e; PHB1
• Locus ID: 5245
• UniProt ID: P35232, A8K401, Q53FV0
• Cytogenetics: 17q21.33
• Summary: 'This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]'
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors