EIF2B2 (NM_014239) Human Mass Spec Standard

CAT#: PH301351

EIF2B2 MS Standard C13 and N15-labeled recombinant protein (NP_055054)

Product Images

Coomassie blue staining of purified EIF2B2 protein (Cat# TP301351). The protein was produced from HEK293T cells transfected with EIF2B2 cDNA clone (Cat# RC201351) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201351
• Predicted MW: 39 kDa
• Protein Sequence:
  Sequence Link

  >RC201351 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTA
  AQPSETTVGNMVRRVLKIIREEYGRLHGRTDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINEL
  LVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKA
  GIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPN
  EEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHV
  L
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_055054
• RefSeq Size: 1557
• RefSeq ORF: 1053
• Synonyms: EIF-2Bbeta; EIF2B
• Locus ID: 8892
• UniProt ID: P49770, Q53XC2
• Cytogenetics: 14q24.3
• Summary: This gene encodes the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation. [provided by RefSeq, Aug 2011]