Apoptosis enhancing nuclease (AEN) (NM_022767) Human Mass Spec Standard

CAT#: PH302285

AEN MS Standard C13 and N15-labeled recombinant protein (NP_073604)

Product Images

Coomassie blue staining of purified AEN protein (Cat# TP302285). The protein was produced from HEK293T cells transfected with AEN cDNA clone (Cat# RC202285) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202285
• Predicted MW: 36.4 kDa
• Protein Sequence:
  Sequence Link

  >RC202285 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVPREAPESAQCLCPSLTIPNAKDVLRKRHKRRSRQHQRFMARKALLQEQGLLSMPPEPGSSPLPTPFGA
  ATATEAASSGKQCLRAGSGSAPCSRRPAPGKASGPLPSKCVAIDCEMVGTGPRGRVSELARCSIVSYHGD
  VLYDKYIRPEMPIADYRTRWSGITRQHMRKAVPFQVAQKEILKLLKGKVVVGHALHNDFQALKYVHPRSQ
  TRDTTYVPNFLSEPGLHTRARVSLKDLALQLLHKKIQVGQHGHSSVEDATTAMELYRLVEVQWEQQEARS
  LWTCPEDREPDSSTDMEQYMEDQYWPDDLAHGSRGGAREAQDRRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_073604
• RefSeq Size: 3134
• RefSeq ORF: 975
• Synonyms: ISG20L1; pp12744
• Locus ID: 64782
• UniProt ID: Q8WTP8
• Cytogenetics: 15q26.1