MED31 (NM_016060) Human Mass Spec Standard

CAT#: PH303047

MED31 MS Standard C13 and N15-labeled recombinant protein (NP_057144)

Product Images

Coomassie blue staining of purified MED31 protein (Cat# TP303047). The protein was produced from HEK293T cells transfected with MED31 cDNA clone (Cat# RC203047) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203047
• Predicted MW: 15.8 kDa
• Protein Sequence:
  Sequence Link

  >RC203047 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKDPEYAKYLKYP
  QCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMRLQQALAEQQQQNNTSGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057144
• RefSeq Size: 1656
• RefSeq ORF: 393
• Synonyms: 3110004H13Rik; CGI-125; Soh1
• Locus ID: 51003
• UniProt ID: Q9Y3C7
• Cytogenetics: 17p13.1
• Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [UniProtKB/Swiss-Prot Function]
• Protein Families: Transcription Factors