DAP13 (NDUFA12) (NM_018838) Human Mass Spec Standard

CAT#: PH303265

NDUFA12 MS Standard C13 and N15-labeled recombinant protein (NP_061326)

Product Images

Coomassie blue staining of purified NDUFA12 protein (Cat# TP303265). The protein was produced from HEK293T cells transfected with NDUFA12 cDNA clone (Cat# RC203265) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203265
• Predicted MW: 17.1 kDa
• Protein Sequence:
  Sequence Link

  >RC203265 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELVQVLKRGLQQITGHGGLRGYLRVFFRTNDAKVGTLVGEDKYGNKYYEDNKQFFGRHRWVVYTTEMNG
  KNTFWDVDGSMVPPEWHRWLHSMTDDPPTTKPLAARKFIWTNHKFNVTGTPEQYVPYSTTRKKIQEWIPP
  STPYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_061326
• RefSeq Size: 592
• RefSeq ORF: 435
• Synonyms: B17.2; DAP13; MC1DN23
• Locus ID: 55967
• UniProt ID: Q9UI09
• Cytogenetics: 12q22
• Summary: This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]